CELL WALL OF PERIDINIUM WESTII A NON CELLULOSIC GLUCAN

被引:41
作者
NEVO, Z
SHARON, N
机构
[1] Department of Biochemistry and Biophysics, The Weizmann Institute of Science, Rehovoth
关键词
D O I
10.1016/0005-2736(69)90099-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cells of the dinoflagellate alga Peridinium westii were collected from water samples, taken from Lake Tiberias, during the period of algal blooms, and were purified by a procedure based on their phototaxis. Clean cell walls were isolated by mechanical disintegraton of the purified whole cells, followed by differential centrifugation, and washing with detergents and organic solvents. The isolated walls were examined by physical, chemical and enzymatic techniques. The major component of the skeletal cell wall (about 95% of its weight was found to be a polymer of d-glucose which differed from cellulose in its X-ray diffraction pattern, solubility in Schweitzer reagent and cadoxen, and other physical and chemical properties. Partial degradation of cell wall glucan by acetolysis and acid hydrolysis afforded a series of cellodextrins (n = 2 to 4), as well as laminaribiose and laminaritriose, indicating that the glucose units are joined by β-(1 → 4)- and β-(1 → 3)-linkages. It has not been established whether the two types of linkages are present in the same polymer or in different polymeric molecules. A pronounced degradation of the cell wall glucan by β-glucanases of snail digestive juice was achieved only when phosphoric acid-treated cell walls were used as a substrate. The relatively low rate of decomposition of the cell wall glucan, in comparison to other known glucans, under natural conditions, seems to be related to its closed structure which serves as a physical barrier for enzyme penetration. © 1969.
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页码:161 / +
页数:1
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