SEQUENCE DETERMINATION OF PEPTIDE BY COMBINED USE OF FLUORESCEINISOTHIOCYANATE AND PHENYLISOTHIOCYANATE

被引：41

作者：

MURAMOTO, K ^{[1
]}

KAWAUCHI, H ^{[1
]}

TUZIMURA, K ^{[1
]}

机构：

[1] KITASATO UNIV,SCH FISHERIES SCI,SAGAMIHARA,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1978年 / 42卷 / 08期

关键词：

D O I：

10.1080/00021369.1978.10863204

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A fluorometric manual technique has been demonstrated for determining the amino acid sequence of a polypeptide, by the combined use of fluoresceinisothiocyanate (FITC) and phenylisothiocyanate (pITC). This method permits continuous assignments of 10 residues with 5.6 nmoles of myoglobin or 23 residues with 45 nmoles. © 1978 Taylor & Francis Group, LLC.

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页码：1559 / 1563

页数：5

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