DISSOLVED PRIMARY AMINE MEASUREMENT BY FLOW-INJECTION ANALYSIS WITH ORTHO-PHTHALDIALDEHYDE - COMPARISON WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：21

作者：

DELMAS, D

FRIKHA, MG

LINLEY, EAS

机构：

[1] Centre de Recherche en Ecologie Marine et Aquaculture de l'Houmeau (CNRS-IFREMER), Case 5

来源：

MARINE CHEMISTRY | 1990年 / 29卷 / 2-3期

关键词：

D O I：

10.1016/0304-4203(90)90010-A

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Dissolved primary amine measurement by flow injection analysis and fluorescence labelling with o-phthaldialdehyde (OPA) has been compared with high-performance liquid chromatography (HPLC). We found a very good agreement between the methods for most of the samples (n > 100, r > 0.85, at 0.1% level). However, for samples where ammonia greatly overbalances dissolved free amino acid (DFAA) concentrations (NH4/DFAA≫10), such as pore waters from reduced environments, the method was invalid. Despite this limitation, the flow injection analysis with OPA appeared to be a promising procedure for primary amine study as very good sensitivity (<0.1 μM) and reproducibility (relative standard deviation <10% at the 0.2-1 μM level) were obtained from analyses which took only 1 min per sample. © 1990.

引用

页码：145 / 154

页数：10

共 27 条

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