DNA ADDUCTS IN HUMANS ENVIRONMENTALLY EXPOSED TO AROMATIC-COMPOUNDS IN AN INDUSTRIAL-AREA OF POLAND

The effect of environmental pollution of DNA adducts in humans was analysed in a highly industralized area of Poland. Coded samples of white blood cells DNA were analysed by 32P-postlabelling and immunoassay from three populations: coke workers, exposed occupationally to high levels of polycyclic aromatic hydrocarbons (PAHs); residents of the towns around cokeries (local controls); and residents from rural Poland (countryside controls). Local controls exhibited adduct levels and patterns similar to those of coke workers, while the levels in rural controls were 2-3 times lower. The results, based on coded samples and two different assays, suggest that environmental pollution is likely to contribute to the adduct levels in local controls. Furthermore, the results show that the levels of aromatic adducts in white blood cell DNA do not linearliy relate to ambient air levels of PAHs but other sources such as food may be important contributors. © 1990 Oxford University Press.