DETECTION OF A NOVEL MINISATELLITE-SPECIFIC DNA-BINDING PROTEIN

被引：60

作者：

COLLICK, A

JEFFREYS, AJ

机构：

[1] Department of Genetics, University of Leicester, Leicester LE1 7RH, University Road

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 03期

关键词：

D O I：

10.1093/nar/18.3.625

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the detection of a ubiquitous DNA-binding protein which appears to interact specifically with tandem-repeated minisatellites. The murine 40kd protein, which we term Msbp-1, was found to be present in all mouse tissues tested. This protein was bound specifically and with high affinity by double-stranded DNA containing a repeat sequence related to the minisatellite 'core' sequence, and binding required the presence of multiple repeat units. Corresponding minisatellite-specific DNA-binding proteins could also be detected in species ranging from Drosophila to man. This analysis represents the first direct evidence that minisatellites can function as a specific recognition signal for an endogenous DNA-binding protein. © 1990 Oxford University Press.

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页码：625 / 629

页数：5

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