A FISSION-YEAST GENE ENCODING A PROTEIN WITH FEATURES OF PROTEIN-TYROSINE-PHOSPHATASES

被引：50

作者：

OTTILIE, S ^{[1
]}

CHERNOFF, J ^{[1
]}

HANNIG, G ^{[1
]}

HOFFMAN, CS ^{[1
]}

ERIKSON, RL ^{[1
]}

机构：

[1] BOSTON COLL,DEPT BIOL,CHESTNUT HILL,MA 02167

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 08期

关键词：

SCHIZOSACCHAROMYCES-POMBE; TYROSINE PHOSPHORYLATION; CONSERVED SEQUENCES; CELL CYCLE CONTROL;

D O I：

10.1073/pnas.88.8.3455

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Degenerate oligonucleotide probes encoding sequences conserved among mammalian protein-tyrosine-phosphatases (PTPases) were used to amplify DNA fragments from a Schizosaccharomyces pombe cDNA library by polymerase chain reaction (PCR) methods. A cloned PCR product predicted peptide sequences similar to those found in PTPases but not identical to any published sequences. A S. pombe gene, designated pypl+, was identified in a cDNA library with this PCR probe, cloned, and sequenced. The sequence of the gene predicted a 550-amino acid protein with M(r) 61,586, which includes amino acid sequences that are highly conserved in mammalian PTPases. Disruption of the pypl+ gene resulted in viable cells. Overexpression of the pypl+ gene in S. pombe permitted detection of a protein of apparent M(r) 63,000.

引用

页码：3455 / 3459

页数：5

共 39 条

[1] THE FISSION YEAST CDC2 CDC13 SUC1 PROTEIN-KINASE - REGULATION OF CATALYTIC ACTIVITY AND NUCLEAR-LOCALIZATION [J].