CURRENT METHODS FOR YAC CLONE CHARACTERIZATION

被引：19

作者：

NELSON, DL

机构：

[1] Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1990年 / 7卷 / 05期

关键词：

D O I：

10.1016/0735-0651(90)90014-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast artificial chromosome (YAC) cloning has allowed isolation of much longer DNA fragments than was previously possible. While this technology makes feasible the isolation of large regions of complex genomes in overlapping cloned segments, it has also required altered methods for manipulation of cloned DNAs. In particular, the isolation of insert sequences from YAC clones has been especially difficult. Methods for characterization of YAC inserts are described and compared. © 1990.

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页码：100 / 106

页数：7

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