EXPRESSION OF CLASS-II MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGEN ON MOUSE SPERM AND ITS ROLES IN FERTILIZATION

被引：31

作者：

MORI, T ^{[1
]}

WU, GM ^{[1
]}

MORI, E ^{[1
]}

SHINDO, Y ^{[1
]}

MORI, N ^{[1
]}

FUKUDA, A ^{[1
]}

MORI, T ^{[1
]}

机构：

[1] KYOTO UNIV,FAC MED,DEPT OBSTET & GYNECOL,KYOTO 606,JAPAN

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 1990年 / 24卷 / 01期

关键词：

in vitro fertilization; MHC antigen; posterior region of sperm;

D O I：

10.1111/j.1600-0897.1990.tb00688.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

ABSTRACT: The expression of class II major histocompatibility complex (MHC) antigen on the membrane of mouse sperm head was clearly demonstrated by indirect immunofluorescence (IIF) test, enzyme immunoassay (EIA), and mixed lymphocyte sperm reaction (MLSR) as well as the examination of its expression at transcriptional levels by nothern dot blotting. Furthermore, the roles of class II MHC antigen of sperm in fertilization were investigated with an in vitro fertilization (IVF) system. The successful ratio of IVF was significantly decreased by treatment of sperm with anticross‐reactive region of class II MHC antigen monoclonal antibody (MAb) but not with antiprivate region MAb. These results were not due to disturbances of sperm mobility by these MAbs. It was strongly suggested that the monomorphic or its related region of class II MHC antigen on sperm played an important role in the recognition between sperm and egg in fertilization. 1990 Munksgaard

引用

页码：9 / 14

页数：6

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