STABILITY PERFORMANCE OF CYNARA-CARDUNCULUS L ACID PROTEASE IN AQUEOUS-ORGANIC BIPHASIC SYSTEMS

被引：11

作者：

BARROS, MT

CARVALHO, MGV

GARCIA, FAP

PIRES, EMV

机构：

[1] UNIV COIMBRA,CTR BIOL CELULAR,P-3000 COIMBRA,PORTUGAL

[2] UNIV COIMBRA,DEPT ENGN QUIM,P-3000 COIMBRA,PORTUGAL

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 03期

关键词：

D O I：

10.1007/BF01023355

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Stability performance of the acid protease of Cynara cardunculus L. in biphasic systems containing ethyl acetate, n-hexane or isooctane was investigated and compared with that of pepsin. Activity retention was higher in the system containing n-hexane. In this system 100% retention was observed up to 144 hours. Pre-saturation of phases was found to increase enzyme stability in the cases of n-hexane and isooctane and to be an absolute requirement in the case of ethyl acetate. The results obtained suggest also that, when dealing with pre-saturated phases, log P cannot be used straightforwardly to predict enzyme stability in biphasic systems.

引用

页码：179 / 184

页数：6

共 13 条

[1] THE MECHANISM OF IRREVERSIBLE ENZYME INACTIVATION AT 100-DEGREES-C [J].