RAPID ESTIMATION OF BACTERIAL ANTIBIOTIC SUSCEPTIBILITY WITH FLOW-CYTOMETRY

被引：70

作者：

MASON, DJ

ALLMAN, R

STARK, JM

LLOYD, D

机构：

[1] VELINDRE HOSP,CLIN ONCOL SERV,CARDIFF CF4 7XL,S GLAM,WALES

[2] UNIV WALES COLL MED,DEPT MED MICROBIOL,CARDIFF CF4 4XN,S GLAM,WALES

[3] UNIV WALES COLL CARDIFF,SCH PURE & APPL BIOL,CARDIFF CF1 3TL,S GLAM,WALES

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1994年 / 176卷

关键词：

FLOW CYTOMETRY; OXONOL DYE; RAPID METHOD; ANTIBIOTIC SUSCEPTIBILITY; ESCHERICHIA COLI; STAPHYLOCOCCUS SPP;

D O I：

10.1111/j.1365-2818.1994.tb03494.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Bacterial antibiotic susceptibility was rapidly estimated for Escherichia coli and Staphylococcus spp. by flow cytometry. This was achieved by measuring the uptake of a negatively charged membrane potential sensitive dye bis-(1,3-dibutyl-barbituric acid) trimethine oxonol and observing changes in low-angle light scatter (excitation light scattered by up to 15 degrees). Estimations of ampicillin, gentamicin and ciprofloxacin susceptibilities were possible within 2-5 h from a plate culture, depending on the species and antibiotic used. This includes the time necessary to establish steady-state growth in liquid culture.

引用

页码：8 / 16

页数：9

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