THE ONTOGENY OF DOPACHROME ISOMERASE ISOZYME PATTERNS IN THE TOBACCO HORNWORM MANDUCA-SEXTA

被引：3

作者：

NELLAIAPPAN, K ^{[1
]}

NICKLAS, G ^{[1
]}

SUGUMARAN, M ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS,DEPT BIOL,BOSTON,MA 02125

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1559

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Melanogenesis in animals is initiated by tyrosinase and augmented by recently discovered dopachrome isomerase. Mushroom tyrosinase embedded 5% polyacrylamide gels are used to specifically stain for dopachrome isomerase activity after electrophoretic separation. Using this new procedure, the ontogenic changes in dopachrome isomerase are demonstrated for the first time in any organism. While there is no isozyme variation within the same stage of development, there are profound variations in isozyme patterns during the developmental stages from egg to adult, indicating differential expression and/or processing of dopachrome isomerase genes to match the metabolic needs at these stages. (C) 1994 Academic Press, Inc.

引用

页码：1072 / 1078

页数：7

共 20 条

[1]

ASO Y, 1984, INSECT BIOCHEM, V14, P463, DOI 10.1016/0020-1790(84)90103-3

[2] TECHNIQUES FOR REARING LABORATORY COLONIES OF TOBACCO HORNWORMS AND PINK BOLLWORMS LEPIDOPTERA-SPHINGIDAE-GELECHIIDAE [J].