HPLC ENANTIOSEPARATION OF DIPEPTIDE AND TRIPEPTIDE ON CYCLODEXTRIN-BONDED STATIONARY PHASES AFTER DERIVATIZATION WITH 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE (AQC)

被引：27

作者：

CHEN, SS ^{[1
]}

PAWLOWSKA, M ^{[1
]}

ARMSTRONG, DW ^{[1
]}

机构：

[1] UNIV MISSOURI,DEPT CHEM,ROLLA,MO 65401

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 03期

关键词：

Liquid chromatography;

D O I：

10.1080/10826079408013155

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Enantiomeric separations were performed on a number of di- and tripeptides after their pre-column derivatization with the fluorescence derizatizing agent 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). It has been demonstrated that the choice of a suitable cyclodextrin bonded phase in conjunction with nonaqueous polar mobile phases offers an effective means to resolve chiral peptides. It was found that the strength of interaction between the cyclodextrin and the solute, and therefore the retention and stereoselectivity, is extremely sensitive to small structural variations of the analyte.

引用

页码：483 / 497

页数：15

共 4 条

[1] Cohen S.A., 1993, TECHNIQUES PROTEIN C, P289
[2] SYNTHESIS OF A FLUORESCENT DERIVATIZING REAGENT, 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE, AND ITS APPLICATION FOR THE ANALYSIS OF HYDROLYSATE AMINO-ACIDS VIA HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
COHEN, SA
MICHAUD, DP
[J]. ANALYTICAL BIOCHEMISTRY, 1993, 211 (02) : 279 - 287
[3] ENANTIOMERIC SEPARATION OF FLUORESCENT, 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE, TAGGED AMINO-ACIDS
PAWLOWSKA, M
CHEN, SS
ARMSTRONG, DW
[J]. JOURNAL OF CHROMATOGRAPHY, 1993, 641 (02): : 257 - 265
[4] EFFICIENT ENANTIOSELECTIVE SEPARATION AND DETERMINATION OF TRACE IMPURITIES IN SECONDARY AMINO-ACIDS (IE, IMINO ACIDS)
ZUKOWSKI, J
PAWLOWSKA, M
ARMSTRONG, DW
[J]. JOURNAL OF CHROMATOGRAPHY, 1992, 623 (01): : 33 - 41

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