TRYPANOSOMA-CRUZI - IMMUNOLOGICAL CROSS-REACTIVITY OF THE MAJOR CYSTEINYL PROTEINASE (CRUZIPAIN) WITH A PARASITE CYTOSOL ACIDIC ANTIGEN (F-IV)

被引：3

作者：

CERBAN, FM

MOTRAN, C

LADERACH, D

GEA, S

GRUPPI, A

VOTTEROCIMA, E

机构：

[1] Inmunologia, Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, 5016 Cordoba, Ciudad Universitaria, Pabellon Argentina, Subsuelo

来源：

ACTA TROPICA | 1994年 / 58卷 / 3-4期

关键词：

TRYPANOSOMA CRUZI; CRUZIPAIN; CYSTEINE PROTEINASE; EPIMASTIGOTE; CROSS-REACTIVITY; ACIDIC ANTIGEN;

D O I：

10.1016/0001-706X(94)90027-2

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

This paper shows that a polyclonal monospecific rabbit antiserum to cruzi cystein, the major T. cruzi cystein proteinase, cross-reacts with a cytosol acidic antigen (F IV) isolated from the epimastigote stage of the same parasite. In addition, antibodies specific for F IV purified from chagasic patient sera or murine anti F IV sera, also react with cruzipain. This was demonstrated by ELISA, DOT-ELISA, native and electrophoretic Immunoblot. These findings suggest that F IV contains an antigen immunologically cross-reactive with cruzipain.

引用

页码：337 / 343

页数：7

共 21 条

[1]

ARNHOLDT ACV, 1993, J IMMUNOL, V151, P3171

[2] DIGESTION OF HUMAN IMMUNOGLOBULIN-G BY THE MAJOR CYSTEINE PROTEINASE (CRUZIPAIN) FROM TRYPANOSOMA-CRUZI [J].

BONTEMPI, E ;

CAZZULO, JJ .

FEMS MICROBIOLOGY LETTERS, 1990, 70 (03) :337-341

[3]

CAMPETELLA O, 1990, FEMS MICROBIOL LETT, V67, P145, DOI [10.1111/j.1574-6968.1990.tb13852.x, 10.1016/0378-1097(90)90184-R]

[4] FURTHER CHARACTERIZATION AND PARTIAL AMINO-ACID SEQUENCE OF A CYSTEINE PROTEINASE FROM TRYPANOSOMA-CRUZI [J].

CAZZULO, JJ ;

COUSO, R ;

RAIMONDI, A ;

WERNSTEDT, C ;

HELLMAN, U .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1989, 33 (01) :33-42

[5] IGG ISOTYPE PROFILES INDUCED IN MICE BY 2 TRYPANOSOMA-CRUZI ELECTRONEGATIVE ANTIGENS [J].

CERBAN, F ;

GRUPPI, A ;

GEA, S ;

VOTTEROCIMA, E .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1991, 96 (01) :35-40

[6] TRYPANOSOMA-CRUZI - INVOLVEMENT OF IGG ISOTYPES IN THE PARASITEMIA CONTROL OF MICE IMMUNIZED WITH PARASITE EXOANTIGENS OF ISOELECTRIC POINT 4.5 [J].

CERBAN, F ;

GRUPPI, A ;

VOTTEROCIMA, E .

EXPERIMENTAL PARASITOLOGY, 1992, 75 (01) :137-145

[7] CHAGAS-DISEASE - IGG ISOTYPES AGAINST TRYPANOSOMA-CRUZI CYTOSOL ACIDIC ANTIGENS IN PATIENTS WITH DIFFERENT DEGREES OF HEART DAMAGE [J].

CERBAN, FM ;

GEA, S ;

MENSO, E ;

VOTTEROCIMA, E .

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1993, 67 (01) :25-30

[8]

EAKIN AE, 1992, J BIOL CHEM, V267, P7411

[9]

GEA S, 1990, J CLIN LAB IMMUNOL, V31, P183

[10] CHAGAS-DISEASE CARDIONEUROPATHY - ASSOCIATION OF ANTI-TRYPANOSOMA-CRUZI AND ANTI-SCIATIC NERVE ANTIBODIES [J].

GEA, S ;

ORDONEZ, P ;

CERBAN, F ;

IOSA, D ;

CHIZZOLINI, C ;

VOTTEROCIMA, E .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1993, 49 (05) :581-588

← 1 2 3 →