DENSITY FRACTIONATION OF HARD TISSUES - APPLICATION OF COULTER COUNTER TO DENSITY-VOLUME DISTRIBUTION OF DRIED BONE POWDERS

A new technique for the density distribution analysis of small quantities of powdered hard tissues is described. The technique uses an electronic particle size and volume analyser (Coulter Counter") to determine the total relative volumes of the powder fractions isolated by centrifugal density fractionation in organic solvent mixtures. Some of the parameters controlling the density distributions of bone powders are examined and the reproducibility of the fractionation and analysis demonstrated. The application of this technique to human bone samples is illustrated and the results discussed. © 1969 Springer-Verlag."