TEMPERATURE-SENSITIVE MUTANT TS O82 OF VESICULAR STOMATITIS-VIRUS .1. RESCUE AT NON-PERMISSIVE TEMPERATURE BY UV-IRRADIATED VIRUS

Temperature-sensitive mutant ts 082 of vesicular stomatitis virus efficiently complements with all the five known groups of ts mutants. This mutant is analyzed by its rescue at nonpermissive temperature with uv-irradiated virus. The existence of two targets of uv light is demonstrated that suggests rescue is mediated through two different complementation processes, gene+ survival and structural protein supply by the uv-irradiated virus. This last point is supported by rescue assays carried out in the absence of simultaneous protein synthesis. These features are also shared by the ts mutants of group II, suggesting that ts 082 might belong to complementation group 11. The temperature-sensitive defect of ts 082 could be located in a domain different from the one which is mutated in the ts mutants previously classified in group II. © 1979.