SUBSTRATE-SPECIFICITY OF ENZYMES FROM PAPAYA CALLUS-CULTURES

被引：3

作者：

MELL, GP

MEDORA, RS

BILDERBACK, DE

机构：

[1] UNIV MONTANA, SCH PHARM, MISSOULA, MT 59812 USA

[2] UNIV MONTANA, COLL ARTS & SCI, MISSOULA, MT 59812 USA

来源：

ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE | 1979年 / 91卷 / 03期

关键词：

D O I：

10.1016/S0044-328X(79)80101-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Optimal protease activity in extracts of callus cultures of papaya [Carica papaya] is obtained by triturating lyophilized callus powder with phosphate buffer at pH 6.0 in a glass mortar; addition of reducing agents to the extraction medium does not increase or stabilize enzyme activity. Casein, casein yellow, azocasein and azoalbumin are good substrates for the proteases; little or no activity was measured with hemoglobin, azocoll, hide powder azure and benzoyl-DL-arginine-p-nitroanilide (BAPA) as substrate.

引用

页码：279 / 282

页数：4