A SPECTROPHOTOMETRIC METHOD FOR CHLOROPLAST ATP-SYNTHETASE ASSAY

被引：1

作者：

KIM, CS ^{[1
]}

JUNG, J ^{[1
]}

机构：

[1] SEOUL NATL UNIV,DEPT AGR CHEM,SUWON 441744,SOUTH KOREA

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1991年 / 53卷 / 03期

关键词：

D O I：

10.1111/j.1751-1097.1991.tb03649.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present work an assay method of chloroplast adenosine triphosphate (ATP) synthetase has been studied in which the production of ATP from thylakoids was monitored spectrophotometrically by the reduction of NADP+ via hexokinase and glucose-6-phosphate dehydrogenase reactions. The interference caused by photosynthetic NADPH production was completely eliminated by use of methyl viologen, a PSI electron acceptor. Under the assay conditions established in this study, the rate of ATP synthesis was directly proportional to the amount of chloroplast ATP synthetase. The method appears reliable, convenient and considerably sensitive. In addition, this technique provides a continuous measurement of ATP production from thylakoids with a limiting adenosine diphosphate concentration.

引用

页码：411 / 414

页数：4

共 23 条

[1]

Allen J. F., 1986, Photosynthesis: energy transduction: a practical approach, P103

[2] COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].