INTERLEUKIN-6 STIMULATES GROWTH OF VASCULAR SMOOTH-MUSCLE CELLS IN A PDGF-DEPENDENT MANNER

被引：213

作者：

IKEDA, U ^{[1
]}

IKEDA, M ^{[1
]}

OOHARA, T ^{[1
]}

OGUCHI, A ^{[1
]}

KAMITANI, T ^{[1
]}

TSURUYA, Y ^{[1
]}

KANO, S ^{[1
]}

机构：

[1] JICHI MED SCH, DEPT CLIN IMMUNOL, MINAMI KAWACHI, TOCHIGI 32904, JAPAN

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 260卷 / 05期

关键词：

PLATELET-DERIVED GROWTH FACTOR; CALCIUM;

D O I：

10.1152/ajpheart.1991.260.5.H1713

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

We have investigated the effect of interleukin 6 (IL-6) on the growth of vascular smooth muscle cells (VSMC) isolated from rat aortas. Murine recombinant IL-6 significantly increased the number of VSMC and stimulated tritiated thymidine incorporation into VSMC in a dose-dependent manner. The IL-6-induced thymidine incorporation into VSMC was totally inhibited by the Ca2+ channel blocker verapamil; however, IL-6 showed no effects on the intracellular Ca2+ level ([Ca2+]i) in VSMC. Antibody against platelet-derived growth factor (PDGF) also totally inhibited the IL-6-induced thymidine uptake. PDGF caused a significant increase in the [Ca2+]i, which was totally inhibited by verapamil. IL-6 mRNA was not detected in unstimulated "quiescent" VSMC, but its expression was stimulated by exposure of VSMC to 10% fetal bovine serum. Immunohistochemical study using anti-PDGF antibody showed that IL-6 stimulated PDGF production in VSMC. These results support the premise that IL-6 is released by VSMC in an autocrine manner and promotes the growth of VSMC via induction of endogenous PDGF production.

引用

页码：H1713 / H1717

页数：5

共 18 条

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