ROUTINE MICROBIOLOGICAL ASSAY FOR CARNITINE ACTIVITY IN BIOLOGICAL-FLUIDS AND TISSUES

被引：7

作者：

BAKER, H ^{[1
]}

DEANGELIS, B ^{[1
]}

BAKER, ER ^{[1
]}

REDDI, AS ^{[1
]}

KHALIL, M ^{[1
]}

FRANK, O ^{[1
]}

机构：

[1] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT MED,NEWARK,NJ 07103

来源：

FOOD CHEMISTRY | 1992年 / 43卷 / 02期

关键词：

D O I：

10.1016/0308-8146(92)90228-T

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A practical method for measuring acid-soluble and total (acid- and alkaline-soluble) carnitine growth activity for a carnitine-specific mutant of the enteric yeast Torulopsis bovina in biological fluids and tissues is described. It grows at 37-degrees-C in a simple, cheap, chemically defined medium; its response threshold to carnitine is 100 pg ml-1. Growth is measured as absorbance units with any turbidometer. Carnitine contents in human and rat fluids and tissues so measured accord with those obtained after multi-step extraction methods, enzyme procedures or radiochemical procedures.

引用

页码：141 / 146

页数：6

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