DETERMINATION OF CAROTENOID-PIGMENTS IN SEVERAL TREE LEAVES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：8

作者：

DELASRIVAS, J

MILICUA, JCG

GOMEZ, R

机构：

[1] Department of Biochemistry, Science Faculty, University of the Basque Country, 48080 Bilbao

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 585卷 / 01期

关键词：

D O I：

10.1016/0021-9673(91)85072-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and simple reversed-phase high-performance liquid chromatographic method has been used to analyse all carotenoid pigments from leaves of five different trees. The pigments were separated on an octadecylsilane radial compression column, using a mobile phase mixtures of acetonitrile, methanol water and ethyl acetate in three isocratic steps. This method resolves all higher plant photosynthetic pigments, carotenoids and also chlorophylls in less than 15 min, while achieving a relatively good separation of trans-lutein-the major carotenoid-from its isomers zeaxanthin and cis-lutein.

引用

页码：168 / 172

页数：5

共 15 条

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