CYCLIC AMP-MEDIATED RELEASE OF PEPTIDE-YY (PYY) FROM THE ISOLATED-PERFUSED RABBIT DISTAL COLON

被引：19

作者：

BALLANTYNE, GH ^{[1
]}

GOLDENRING, JR ^{[1
]}

SAVOCA, PE ^{[1
]}

KRANZ, HK ^{[1
]}

ADRIAN, TE ^{[1
]}

BILCHIK, AJ ^{[1
]}

MODLIN, IM ^{[1
]}

机构：

[1] W HAVEN DVA MED CTR,W HAVEN VA MED CTR,NEW HAVEN,CT

来源：

REGULATORY PEPTIDES | 1993年 / 47卷 / 02期

关键词：

VASOACTIVE INTESTINAL PEPTIDE; SUBSTANCE-P; CARBACHOL; CHOLERA TOXIN; TETRODOTOXIN; A23187; GUT HORMONE; NEUROPEPTIDE; RABBIT;

D O I：

10.1016/0167-0115(93)90415-5

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have investigated the role of the colonic nervous system in regulating the release of peptide YY (PYY) from the isolated perfused rabbit distal colon. In addition, we have studied the role of cAMP- and Ca2+-dependent mechanisms in mediating the release of PYY. We investigated three agents which stimulate increases in intracellular cAMP (vasoactive intestinal polypeptide (VIP), cholera toxin, and forskolin) and three agents which raise intracellular Ca2+ concentrations (substance P, carbachol, and the calcium ionophore A23187). The three cAMP-dependent agents, VIP (10(-7) M and 3.10(-7) M), cholera toxin (100 mug), and forskolin (10(-6) M and 10(-5) M) significantly stimulated release of PYY (P < 0.05). Tetrodotoxin (3.10(-6) M) did not alter forskolin (10(-5) M) stimulated release of PYY. Substance P (10(-9) M and 10(-8) M), carbachol (10(-5) M), and A23187 (10(-6) M) failed to stimulate the release of PYY. These results suggest that the colonic neurotransmitter VIP participates in the modulation of PYY release from rabbit distal colons. Further, these studies suggest that release of PYY is mediated by cAMP-dependent pathways.

引用

页码：117 / 126

页数：10

共 31 条

[1] EFFECT OF PEPTIDE-YY ON GASTRIC, PANCREATIC, AND BILIARY FUNCTION IN HUMANS [J].