PURIFICATION AND CHARACTERIZATION OF DNA-POLYMERASES FROM PLASMODIUM-FALCIPARUM

被引：16

作者：

CHAVALITSHEWINKOON, P

DEVRIES, E

STAM, JG

FRANSSEN, FFJ

VANDERVLIET, PC

OVERDULVE, JP

机构：

[1] UNIV UTRECHT,INST INFECT DIS & IMMUNOL,DEPT PARASITOL & TROP VET MED,POB 80165,3508 TD UTRECHT,NETHERLANDS

[2] UNIV UTRECHT,PHYSIOL CHEM LAB,3508 TD UTRECHT,NETHERLANDS

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 61卷 / 02期

关键词：

DNA POLYMERASE; PLASMODIUM-FALCIPARUM; MALARIA; APHIDICOLIN; INHIBITION OF MALARIAL ENZYMES;

D O I：

10.1016/0166-6851(93)90070-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fractionation of Plasmodium falciparum cellular extracts by fast protein liquid chromatography (FPLC) identified at least two different DNA polymerases. An aphidicolin-sensitive activity co-purified with a primase activity. This, in combination with other characteristics (processivity, sensitivity to other inhibitors), most likely classifies this enzyme as an alpha-like DNA polymerase. It was, however, relatively resistant to N2-(p-n-butylphenyl)deoxyguanosine 5'-triphosphate (IC50 = 6.6 muM) and differs in this aspect from the host homologue, possibly indicating structural differences between host and parasite DNA polymerase alpha. The other DNA polymerase matched eukaryotic DNA polymerase gamma in all properties tested.

引用

页码：243 / 253

页数：11

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