CONSTRUCTION OF A DEFECTIVE ADENOVIRUS VECTOR EXPRESSING THE PSEUDORABIES VIRUS GLYCOPROTEIN GP50 AND ITS USE AS A LIVE VACCINE

被引：77

作者：

ELOIT, M ^{[1
]}

GILARDIHEBENSTREIT, P ^{[1
]}

TOMA, B ^{[1
]}

PERRICAUDET, M ^{[1
]}

机构：

[1] INST GUSTAVE ROUSSY,CNRS,UA 1301,F-94800 VILLEJUIF,FRANCE

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-10-2425

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene encoding the pseudorabies virus glycoprotein gp50 was cloned at the very left end of the genome of adenovirus type 5 to give a recombinant adenovirus (Ad-gp50) defective for the E1A gene. Ad-gp50 expressed high levels of gp50 in cells which either complemented (293 cells) or did not complement (Vero and HeLa cells) the E1A gene. Surprisingly, over an extended period, higher levels of gp50 were produced in HeLa cells which lack the E1A gene. Rabbits and mice inoculated with Ad-gp50 showed a strong antibody response against gp50. Some of them were protected from a virulent challenge with pseudorabies virus.

引用

页码：2425 / 2431

页数：7

共 31 条

[1]

BAER GM, 1989, AM J VET RES, V50, P836

[2] INVITRO AND INVIVO SYNTHESIS OF THE HEPATITIS-B VIRUS SURFACE-ANTIGEN AND OF THE RECEPTOR FOR POLYMERIZED HUMAN-SERUM ALBUMIN FROM RECOMBINANT HUMAN ADENOVIRUSES [J].

BALLAY, A ;

LEVRERO, M ;

BUENDIA, MA ;

TIOLLAIS, P ;

PERRICAUDET, M .

EMBO JOURNAL, 1985, 4 (13B) :3861-3865

[3]

BALLAY A, 1987, UCLA S MOL CELLULAR, V70, P481

[4] SPLICED SEGMENTS AT 5' TERMINUS OF ADENOVIRUS 2 LATE MESSENGER-RNA [J].

BERGET, SM ;

MOORE, C ;

SHARP, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3171-3175

[5]

BERKNER KL, 1988, BIOTECHNIQUES, V6, P616

[6] INOCULATION OF PIGS WITH ADENOVIRUSES OF MAN [J].

BETTS, AO ;

LAMONT, PH ;

JENNINGS, AR ;

PAGE, Z .

NATURE, 1962, 193 (4810) :45-&

[7]

CHAPPNIS G, 1989, VACCINATION CONTROL, P67

[8]

DERMOTT MR, 1989, VIROLOGY, V169, P244

[9] DEVELOPMENT OF AN ELISA TO DIFFERENTIATE BETWEEN ANIMALS EITHER VACCINATED WITH OR INFECTED BY AUJESZKYS DISEASE VIRUS [J].

ELOIT, M ;

FARGEAUD, D ;

VANNIER, P ;

TOMA, B .

VETERINARY RECORD, 1989, 124 (04) :91-94

[10] IDENTIFICATION OF THE PSEUDORABIES VIRUS GLYCOPROTEIN-GP50 AS A MAJOR TARGET OF NEUTRALIZING ANTIBODIES [J].

ELOIT, M ;

FARGEAUD, D ;

LHARIDON, R ;

TOMA, B .

ARCHIVES OF VIROLOGY, 1988, 99 (1-2) :45-56

← 1 2 3 4 →