ENZYMATIC-SYNTHESIS OF LEU-ENKEPHALIN AND MET-ENKEPHALIN

被引：41

作者：

KULLMANN, W

机构：

[1] Max-Planck-Institut für biophysikalische Chemie, D-3400 Göttingen, Am Faßberg

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)91577-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The protease-catalyzed synthesis of Leu- and Met-enkephalin is reported. Each peptide bond of the endogeneous opiate-pentapeptides was formed either by papain or α-chymotrypsin catalysis. N-acyl amino acids and peptides or their ester derivatives served as substrates whereas amino acid and peptide phenylhydrazides were used as nucleophiles. The free pentapeptides exhibited naloxone-reversible opiate-like activity in guinea-pig ileum and mouse vas deferens assays. The present study suggests the usefulness of enzymic peptide synthesis which allows rapid preparation of homogeneous compounds with high optical purity. © 1979.

引用

页码：693 / 698

页数：6

共 16 条

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