A KINETIC-STUDY OF THE CATALYZED OXIDATION OF 1',3-DIMETHYLFERROCENE ETHYLAMINE BY CYTOCHROME-C PEROXIDASE

被引：20

作者：

COOPER, JM

BANNISTER, JV

MCNEIL, CJ

机构：

[1] UNIV GLASGOW,DEPT ELECTR & ELECT ENGN,GLASGOW G12 8QQ,SCOTLAND

[2] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT CLIN BIOCHEM,NEWCASTLE TYNE NE2 4HH,ENGLAND

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1991年 / 312卷 / 1-2期

关键词：

D O I：

10.1016/0022-0728(91)85150-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The kinetics of the catalysed oxidation of 1',3-dimethylferrocene ethylamine by cytochrome c peroxidase was studied in the presence of two hydroperoxide substrates using cyclic voltammetry. The second order rate constants for the reduction of oxidised CcP were determined as 3.57 X 10(6) dm3 mol-1 s-1 in the presence of hydrogen peroxide and 5.00 X 10(5) dm3 mol-1 s-1 in the presence of m-chloroperoxybenzoic acid. Hydrogen peroxide therefore proved to be a more efficient electron acceptor than m-chloroperoxybenzoic acid. The relative rates of reaction of the two substrates were found to be similar to those for the immobilised enzyme. In addition we have also presented some preliminary results for the coupling of a range of other ferrocene derivatives with CcP.

引用

页码：155 / 163

页数：9

共 12 条

[1] FAST INTERFACIAL ELECTRON-TRANSFER BETWEEN CYTOCHROME-C PEROXIDASE AND GRAPHITE-ELECTRODES PROMOTED BY AMINOGLYCOSIDES - NOVEL ELECTROENZYMIC CATALYSIS OF H2O2 REDUCTION [J].