SEQUENCE-SPECIFIC H-1-NMR ASSIGNMENT AND DETERMINATION OF THE SECONDARY STRUCTURE OF BOVINE HEART FATTY-ACID-BINDING PROTEIN

被引：24

作者：

LUCKE, C

LASSEN, D

KREIENKAMP, HJ

SPENER, F

RUTERJANS, H

机构：

[1] UNIV FRANKFURT,INST BIOPHYS CHEM,THEODOR STERN KAI 7 HAUS 75A,W-6000 FRANKFURT,GERMANY

[2] UNIV MUNSTER,INST BIOCHEM,W-4400 MUNSTER,GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 210卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1992.tb17494.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nearly complete sequence-specific H-1 resonance assignment of the pI = 4.9 isoform of cytosolic 15-kDa fatty-acid-binding protein from bovine heart (H-FABP(c)) by homonuclear two-dimensional NMR spectroscopy is presented. Regular secondary structure elements were identified from NOE spectra and the sequence locations of slowly exchanging backbone amide protons. The molecular structure of the protein was found to consist mainly of ten antiparallel beta-strands and two short alpha-helices. The data presented here for the first time for a hydrophobic molecule transporter of the fatty-acid-binding protein type is the basis for a complete tertiary structure determination currently in progress.

引用

收藏

页码：901 / 910

页数：10

相关论文

共 24 条

[1] THE CELLULAR FATTY-ACID BINDING-PROTEINS - ASPECTS OF STRUCTURE, REGULATION, AND FUNCTION [J].

BASS, NM .

INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1988, 111 :143-184

[2] FATTY ACID-BINDING PROTEINS .9. CLONING OF A FULL-LENGTH COMPLEMENTARY-DNA FOR FATTY-ACID-BINDING PROTEIN FROM BOVINE HEART [J].

BILLICH, S ;

WISSEL, T ;

KRATZIN, H ;

HAHN, U ;

HAGENHOFF, B ;

LEZIUS, AG ;

SPENER, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 175 (03) :549-556

[3] A RADIOCHEMICAL PROCEDURE FOR THE ASSAY OF FATTY-ACID BINDING BY PROTEINS [J].

GLATZ, JFC ;

VEERKAMP, JH .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :89-95

[4] ANALYZING THE STRUCTURES, FUNCTIONS AND EVOLUTION OF 2 ABUNDANT GASTROINTESTINAL FATTY-ACID BINDING-PROTEINS WITH RECOMBINANT DNA AND COMPUTATIONAL TECHNIQUES [J].

GORDON, JI ;

LOWE, JB .

CHEMISTRY AND PHYSICS OF LIPIDS, 1985, 38 (1-2) :137-158

[5] FATTY-ACID-BINDING PROTEINS - OCCURRENCE OF 2 FATTY-ACID-BINDING PROTEINS IN BOVINE LIVER CYTOSOL AND THEIR BINDING OF FATTY-ACIDS, CHOLESTEROL, AND OTHER LIPOPHILIC LIGANDS [J].

HAUNERLAND, N ;

JAGSCHIES, G ;

SCHULENBERG, H ;

SPENER, F .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1984, 365 (03) :365-376

[6] FATTY-ACID BINDING-PROTEIN IN FLIGHT-MUSCLE OF THE LOCUST, SCHISTOCERCA-GREGARIA [J].

HAUNERLAND, NH ;

CHISHOLM, JM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1047 (03) :233-238

[7] BOVINE FATTY-ACID BINDING-PROTEINS .5. ISOLATION AND CHARACTERIZATION OF 2 CARDIAC FATTY-ACID BINDING-PROTEINS THAT ARE DISTINCT FROM CORRESPONDING HEPATIC PROTEINS [J].

JAGSCHIES, G ;

REERS, M ;

UNTERBERG, C ;

SPENER, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 152 (03) :537-545

[8] THE 3-DIMENSIONAL STRUCTURE OF P2 MYELIN PROTEIN [J].

JONES, TA ;

BERGFORS, T ;

SEDZIK, J ;

UNGE, T .

EMBO JOURNAL, 1988, 7 (06) :1597-1604

[9] 3-DIMENSIONAL STRUCTURE OF FATTY-ACID-BINDING PROTEIN FROM BOVINE HEART [J].

MULLERFAHRNOW, A ;

EGNER, U ;

JONES, TA ;

RUDEL, H ;

SPENER, F ;

SAENGER, W .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 199 (02) :271-276

[10]

OCKNER RK, 1982, J BIOL CHEM, V257, P7872