PURIFICATION AND CHARACTERIZATION OF GIANT EMPTY PROHEADS FROM PACKAGING-DEFECTIVE 23PTG MUTANTS OF BACTERIOPHAGE-T4

被引：4

作者：

RAO, VB

LEFFERS, G

机构：

[1] Department of Biology, Institute for Biomolecular Studies, The Catholic University of America, Washington

来源：

VIROLOGY | 1993年 / 196卷 / 02期

关键词：

D O I：

10.1006/viro.1993.1554

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We constructed packaging-defective gene 23ptg mutants of bacteriophage T4 that produced giant proheads of varying lengths. The giant proheads constituted up to 20% of the total proheads. A purification protocol was developed to prepare large quantities of highly pure giant proheads. The purified giant proheads appeared to package both mature T4 DNA and concatemeric AD10 plasmid DNA in vitro. © 1993 by Academic Press, Inc.

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页码：896 / 899

页数：4

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