EXTENSIVE AND EQUIVALENT REPAIR IN BOTH RADIATION-RESISTANT AND RADIATION-SENSITIVE ESCHERICHIA-COLI DETERMINED BY A DNA-UNWINDING TECHNIQUE

The extent of strand breakage and repair in irradiated E. coli B/r and BS-1 was studied using a DNA-unwinding technique in denaturing conditions of weak alkali. Although these two strains show widely different responses to the lethal effects of ionizing radiation, they both have an equal capacity to repair radiation-induced breaks in DNA. Oxygen enhancement ratios for the killing of B/r and BS-1 were respectively 4 and 2; but after repair in non-nutrient or nutrient post-irradiation conditions, the oxygen enhancement values for the residual strand breaks were always the same for the two strains. The equal abilities of E. coli B/r and E. coli BS-1 to remove the strand breaks measured by this weak-alkali technique leads us to suggest that some other type of damage to either DNA or another macromolecule may play a major role in determining whether or not the cells survive to proliferate. © 1978 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.