MODIFICATION OF THE POLARON SPUTTER-COATER UNIT FOR GLOW-DISCHARGE ACTIVATION OF CARBON SUPPORT FILMS

被引：39

作者：

BENADA, O

POKORNY, V

机构：

来源：

JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE | 1990年 / 16卷 / 03期

关键词：

Carbon support films; Glowdischarge activation; Polaron sputtercoater;

D O I：

10.1002/jemt.1060160304

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a modification of the Polaron sputtercoater unit series 11 HD enabling activation of carbon support films for electron microscopy of macromolecules and macromolecular assemblies. The modification is simple and the device can be used in two modes, for sputtercoating of SEM samples and for glowdischarge activation of carbon support films. Examples of proteinfree spreading of DNA and negative staining of bacteriophage particles on activated carbon support films are presented. Copyright1990 WileyLiss, Inc.

引用

页码：235 / 239

页数：5

共 7 条

[1] A GLOW-DISCHARGE UNIT TO RENDER ELECTRON-MICROSCOPE GRIDS AND OTHER SURFACES HYDROPHILIC [J].