MODE OF ACTION OF TRICHOTHECIN ON MULTIPLICATION OF NEWCASTLE DISEASE VIRUS IN CULTURED CELLS (STUDIES ON ANTIVIRAL AND ANTITUMOR ANTIBIOTICS .17.)

Trichothecin (1.6 mcg/ml) completely inhibited multiplication of Newcastle disease virus in cultures of chick embryo fibroblasts infected with 50 plaque forming units cell; while at lower concentrations, where complete inhibition was not observed, a dose-response effect was shown by a lengthening of the lag time before the beginning of hemagglutinin synthesis. Trichothecin had no effect on free virus particles or on viral adsorption to host cells. The antibiotic exerted its activity whenever it was added during a viral growth cycle, and the inhibitory action was reversed upon removal of trichothecin by washing. Protein synthesis was promptly suppressed after addition of trichothecin (3.0 mcg/ml), but actinomycin D insensitive RNA synthesis proceeded in the presence of the antibiotic. The RNA was analysed on a methylated albumin-kiselguhr column. © 1969, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.