ANALYSIS OF FATTY-ACIDS FROM HUMAN LIPIDS BY GAS-CHROMATOGRAPHY

被引：62

作者：

HAAN, GJ ^{[1
]}

VANDERHEIDE, S ^{[1
]}

WOLTHERS, BG ^{[1
]}

机构：

[1] STATE UNIV GRONINGEN HOSP, CENT LAB CLIN CHEM, GRONINGEN, NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 162卷 / 03期

关键词：

D O I：

10.1016/S0378-4347(00)81513-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, quantitative method is described for the analysis of fatty acids from human lipids, namely serum lipids and lipids from adipose tissue biopsies. The method includes extraction of serum lipids with chloroform-methanol, hydrolysis with tetramethylammonium hydroxide, methylation with methyl iodide and N,N-dimethylformamide and gas chromatographic analysis on a Supelcoport SP-2330 column. Fat biopsies are analysed without extraction. Optimal hydrolysis conditions have been investigated. © 1979.

引用

页码：261 / 271

页数：11

共 11 条

[1] GEIGY SA, 1968, WISSENSCHAFTLICHE TA, P600
[2] GREELEY RH, 1974, J CHROMATOGR, V88, P229
[3] ROUTINE CHROMATOGRAPHY OF SIMPLE LIPIDS AND THEIR CONSTITUENTS
KUKSIS, A
[J]. JOURNAL OF CHROMATOGRAPHY, 1977, 143 (01): : 3 - 30
[4] LINOLEATE AND FATTY-ACID PATTERNS OF SERUM-LIPIDS IN MULTIPLE-SCLEROSIS AND OTHER DISEASES
LOVE, WC
CASHELL, A
REYNOLDS, M
CALLAGHA.N
[J]. BMJ-BRITISH MEDICAL JOURNAL, 1974, 2 (5922): : 18 - 21
[5] ROSENLUND ML, 1977, PEDIATRICS, V59, P428
[6] SCHENK H, 1960, ANAL CHEM, V32, P1412
[7] POLYUNSATURATED FATTY-ACIDS AND TOCOPHEROL LEVELS IN PATIENTS WITH CYSTIC-FIBROSIS
UNDERWOOD, BA
DENNING, CR
NAVAB, M
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1972, 203 (DEC18) : 237 - 247
[8] VANGENT CM, 1968, Z ANAL CHEM FRESENIU, V236, P344
[9] WAGENER H, 1967, LIPIDS LIPIDOSES, P197
[10] WEAST RC, 1968, HDB CHEM PHYSICS, pD190

← 1 2 →