NATURE OF THE RANDOM EXPERIMENTAL ERROR ENCOUNTERED WHEN ACETYLCHOLINE HYDROLASE AND ALCOHOL-DEHYDROGENASE ARE ASSAYED

Analysis of replicate initial velocities showed that (1) the random errors in the velocities were usually not normally distributed, and (2) their magnitude increased with that of the velocity. Consequently equations should be fitted to data by weighted least-squares or by nonparametric methods. It is also demonstrated that initial velocities derived graphically from curvilinear time courses may be underestimates, and that a numerical method may be used instead. © 1979.