FUNCTIONAL PRESERVATION OF THE MAMMALIAN KIDNEY .5. PHARMACOKINETICS OF DIMETHYL-SULFOXIDE (1.4M) IN KIDNEYS (RABBIT AND DOG) PERFUSED AT 37-DEGREES-C, 25-DEGREES-C OR 10-DEGREES-C FOLLOWED BY TRANSPLANTATION (DOG)

Rabbit and dog kidneys were perfused for 30 min at 37°C with 1.4 M [3H]Me2SO in a K+-Mg2+-rich perfusate. Subsequently the kidneys were perfused for 30 min with Me2SO-free perfusate. The rate of Me2SO uptake and washout as well as Me2SO distribution in the tissue were determined. It was found that equilibrium conditions were achieved within 30 min for both uptake and washout with T M ratios approaching 1.0. The amount of Me2SO in the cortex and medulla of rabbit kidneys was not significantly different. The same experiment was repeated with dog kidneys at 25 and 10°C. At these lower temperatures the rate of uptake and washout was significantly less, but the final concentration achieved within 30 min was the same as at 37°C. Dog kidneys flushed with a K+-Mg2+-rich solution, with or without 1.4 M dimethyl sulfoxide (Me2SO), were kept at 10°C, then reimplanted in the autologous host, and an immediate contralateral nephrectomy was performed. Of the dogs receiving kidneys treated with Me2SO-free solution, 86% survived; of the dogs receiving Me2SO-treated kidneys, 75% survived. Dog kidneys were perfused for 30 min with a K+-Mg2+-rich solution, with or without 1.4 M Me2SO, at 25 or 37°C. All kidneys were then perfused for 30 min with a Me2SO-free solution at the same temperature used for the first perfusion. All kidneys were then reimplanted in the autologous host and an immediate contralateral nephrectomy was performed. Of the dogs receiving kidneys perfused at 25°C with Me2SO-free solution, 43% survived; of the dogs receiving kidneys perfused with Me2SO, 42% survived. Dog kidneys were also treated at 37°C in a manner similar to those at 25°C. Of the dogs receiving kidneys perfused at 37°C with Me2SO-free solution, 80% survived; of the dogs receiving kidneys perfused with Me2SO, 67% survived. Other results indicate that perfusion with a closed circuit is superior to perfusion with an open circuit. Also, gradual administration and washout of Me2SO gives better renal survival than rapid changes in Me2SO concentration. © 1979.