ULTRASENSITIVE DETERMINATION OF EUROPIUM USING MICROSECOND TIME-RESOLVED SPECTROFLUORIMETRY

被引：10

作者：

CHRISTOPOULOS, TK

DIAMANDIS, EP

机构：

[1] TORONTO WESTERN HOSP,DEPT CLIN BIOCHEM,399 BATHURST ST,TORONTO M5T 2S8,ONTARIO,CANADA

[2] UNIV TORONTO,DEPT CLIN BIOCHEM,TORONTO M5G 1L5,ONTARIO,CANADA

来源：

ANALYST | 1991年 / 116卷 / 06期

关键词：

LANTHANIDE-ION CHELATES; TIME-RESOLVED SPECTROFLUORIMETRY; EUROPIUM; ULTRASENSITIVE ASSAY; TRACE METAL DETERMINATION;

D O I：

10.1039/an9911600627

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

4,7-Bis(chlorosulphophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) is proposed as a simple, highly sensitive and specific reagent for the ultrasensitive determination of Eu3+ with microsecond time-resolved spectrofluorimetry. The optimum reaction pH was 9.0 in a glycine buffer. Detection limit for EU3+ was 3 x 10(-13) mol l-1. No major interferences from other metals were found when the interferent was present in a 500-fold molar excess. Negative interference was observed at high (> 1 X 10(-3) mol l-1) concentrations of Fe3+ and Ca2+ presumably through the formation of non-fluorescent complexes with BCPDA.

引用

页码：627 / 630

页数：4

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