DEVELOPMENT AND CHARACTERIZATION OF MURINE MONOCLONAL-ANTIBODIES TO THE LATENCY-ASSOCIATED PEPTIDE OF TRANSFORMING GROWTH-FACTOR BETA(1)

被引：21

作者：

HONGO, JAS

MORAWORMS, M

LUCAS, C

FENDLY, BM

机构：

[1] Dept. of Bioanalytical Technology, Genentech, Inc., South San Francisco, CA 94080

来源：

HYBRIDOMA | 1995年 / 14卷 / 03期

关键词：

D O I：

10.1089/hyb.1995.14.253

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transforming growth factor beta (TGF-beta) is a multifunctional peptide that controls proliferation, differentiation, and other functions in a variety of cell types, Transforming growth factor beta activities have been implicated in a variety of diseased states including arthritis, prostate cancer, and AIDS, and in the repair of tissue injury caused by trauma, burns, and surgery, We describe the development and characterization of novel murine monoclonal antibodies (MAbs) to the latency-associated peptide (LAP) of TGF-beta(1), and the subsequent development of an ELISA for the detection and quantitation of TGF-beta(1)-LAP in buffer and serum matrices, Fusion of immune splenocytes with myeloma cells yielded 576 hybridomas, 110 of which were antibody secreting. Five were selected for extensive characterization. Clinically, the MAbs described here should be valuable for studying potentially abnormal production and/or function of the LAP, and its relationship to TGF-beta.

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页码：253 / 260

页数：8

相关论文

共 39 条

[1] IMMUNOASSAY FOR THE DETECTION OF ESCHERICHIA-COLI PROTEINS IN RECOMBINANT-DNA DERIVED HUMAN GROWTH-HORMONE [J].

ANICETTI, VR ;

FEHSKENS, EF ;

REED, BR ;

CHEN, AB ;

MOORE, P ;

GEIER, MD ;

JONES, AJS .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 91 (02) :213-224

[2] LATENT FORMS OF TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) DERIVED FROM BONE CULTURES - IDENTIFICATION OF A NATURALLY-OCCURRING 100-KDA COMPLEX WITH SIMILARITY TO RECOMBINANT LATENT TGF-BETA [J].

BONEWALD, LF ;

WAKEFIELD, L ;

OREFFO, ROC ;

ESCOBEDO, A ;

TWARDZIK, DR ;

MUNDY, GR .

MOLECULAR ENDOCRINOLOGY, 1991, 5 (06) :741-751

[3] Physicochemical Activation of Recombinant Latent Transforming Growth Factor-beta's 1, 2, and 3 [J].

Brownh, Peter D. ;

Wakefiel, Lalage M. ;

Levinson, Arthur D. ;

Sporn, Michael B. .

GROWTH FACTORS, 1990, 3 (01) :35-43

[4]

Cann J R, 1979, Methods Enzymol, V61, P142

[5]

DASCH JR, 1989, J IMMUNOL, V142, P1536

[6] A NEW TYPE OF TRANSFORMING GROWTH FACTOR-BETA, TGF-BETA-3 [J].

DERYNCK, R ;

LINDQUIST, PB ;

LEE, A ;

WEN, D ;

TAMM, J ;

GRAYCAR, JL ;

RHEE, L ;

MASON, AJ ;

MILLER, DA ;

COFFEY, RJ ;

MOSES, HL ;

CHEN, EY .

EMBO JOURNAL, 1988, 7 (12) :3737-3743

[7] ALTERED RECOGNITION OF SURFACE-ADSORBED COMPARED TO ANTIGEN-BOUND ANTIBODIES IN THE ELISA [J].

DIERKS, SE ;

BUTLER, JE ;

RICHERSON, HB .

MOLECULAR IMMUNOLOGY, 1986, 23 (04) :403-411

[8] MEASUREMENTS OF THE TRUE AFFINITY CONSTANT IN SOLUTION OF ANTIGEN-ANTIBODY COMPLEXES BY ENZYME-LINKED IMMUNOSORBENT-ASSAY [J].

FRIGUET, B ;

CHAFFOTTE, AF ;

DJAVADIOHANIANCE, L ;

GOLDBERG, ME .

JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 77 (02) :305-319

[9] TYPE-1 TRANSFORMING GROWTH-FACTOR-BETA - AMPLIFIED EXPRESSION AND SECRETION OF MATURE AND PRECURSOR POLYPEPTIDES IN CHINESE-HAMSTER OVARY CELLS [J].

GENTRY, LE ;

WEBB, NR ;

LIM, GJ ;

BRUNNER, AM ;

RANCHALIS, JE ;

TWARDZIK, DR ;

LIOUBIN, MN ;

MARQUARDT, H ;

PURCHIO, AF .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (10) :3418-3427

[10] THE EFFECT OF PREINJECTION OF MICE WITH PRISTANE ON ASCITES TUMOR-FORMATION AND MONOCLONAL-ANTIBODY PRODUCTION [J].

HOOGENRAAD, N ;

HELMAN, T ;

HOOGENRAAD, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 61 (03) :317-320

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