SPERMATIDS AS MALE GAMETES

被引：21

作者：

OGURA, A ^{[1
]}

YANAGIMACHI, R ^{[1
]}

机构：

[1] UNIV HAWAII,SCH MED,DEPT ANAT & REPROD BIOL,HONOLULU,HI 96822

来源：

REPRODUCTION FERTILITY AND DEVELOPMENT | 1995年 / 7卷 / 02期

基金：

美国国家卫生研究院;

关键词：

IN VITRO FERTILIZATION; SPERMATOGENIC CELLS; ICSI; ELECTROFUSION;

D O I：

10.1071/RD9950155

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intracytoplasmic sperm injection (ICSI) is becoming increasingly popular in human infertility clinics as an efficient method for the treatment of male infertility. It is proposed that spermatids can be used as substitutes for spermatozoa if men are unable to produce sperm in their testes. At least in the hamster and mouse, the nuclei of round spermatids were capable of participating in syngamy when incorporated into homologous mature oocytes either by microsurgical ICSI or electrofusion. Normal mouse offspring were born after electrofusion of oocytes with round spermatids. When culture in vitro of spermatogonia and spermatocytes is perfected, then spermatids, transforming spermatids and spermatozoa will all be able to be used as male gametes.

引用

页码：155 / 159

页数：5

共 16 条

[1] HYPOTAURINE REQUIREMENT FOR INVITRO DEVELOPMENT OF GOLDEN-HAMSTER ONE-CELL EMBRYOS INTO MORULAE AND BLASTOCYSTS, AND PRODUCTION OF TERM OFFSPRING FROM INVITRO-FERTILIZED OVA [J].

BARNETT, DK ;

BAVISTER, BD .

BIOLOGY OF REPRODUCTION, 1992, 47 (02) :297-304

[2]

CHANG DC, 1992, GUIDE ELECTROPORATIO, P1

[3]

DUPAN RCM, 1993, FERTIL STERIL, V60, P937

[4] DNA-SYNTHESIS FOLLOWING MICROINJECTION OF HETEROLOGOUS SPERM AND SOMATIC-CELL NUCLEI INTO HAMSTER OOCYTES [J].

NAISH, SJ ;

PERREAULT, SD ;

ZIRKIN, BR .

GAMETE RESEARCH, 1987, 18 (02) :109-120

[5] ROUND SPERMATID NUCLEI INJECTED INTO HAMSTER OOCYTES FORM PRONUCLEI AND PARTICIPATE IN SYNGAMY [J].

OGURA, A ;

YANAGIMACHI, R .

BIOLOGY OF REPRODUCTION, 1993, 48 (02) :219-225

[6] BIRTH OF NORMAL YOUNG AFTER ELECTROFUSION OF MOUSE OOCYTES WITH ROUND SPERMATIDS [J].

OGURA, A ;

MATSUDA, J ;

YANAGIMACHI, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (16) :7460-7462

[7]

Ogura A, 1993, ZYGOTE, V1, P1

[8] PREGNANCIES AFTER INTRACYTOPLASMIC INJECTION OF SINGLE SPERMATOZOON INTO AN OOCYTE [J].

PALERMO, G ;

JORIS, H ;

DEVROEY, P ;

VANSTEIRTEGHEM, AC .

LANCET, 1992, 340 (8810) :17-18

[9] TRANSMEIOTIC DIFFERENTIATION OF MALE GERM-CELLS IN CULTURE [J].

RASSOULZADEGAN, M ;

PAQUISFLUCKLINGER, V ;

BERTINO, B ;

SAGE, J ;

JASIN, M ;

MIYAGAWA, K ;

VANHEYNINGEN, V ;

BESMER, P ;

CUZIN, F .

CELL, 1993, 75 (05) :997-1006

[10] CENTRIOLES IN THE BEGINNING OF HUMAN-DEVELOPMENT [J].

SATHANANTHAN, AH ;

KOLA, I ;

OSBORNE, J ;

TROUNSON, A ;

NG, SC ;

BONGSO, A ;

RATNAM, SS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (11) :4806-4810

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