PRODUCTION AND TRANSFORMATION OF CONIDIA OF VENTURIA-INAEQUALIS

被引：51

作者：

PARKER, DM

HILBER, UW

BODMER, M

SMITH, FD

YAO, C

KOLLER, W

机构：

[1] SWISS FED RES STN, DEPT PLANT PATHOL, CH-8820 WADENSWIL, SWITZERLAND

[2] CORNELL UNIV, NEW YORK STATE AGR EXPT STN, DEPT HORT SCI, GENEVA, NY 14456 USA

来源：

PHYTOPATHOLOGY | 1995年 / 85卷 / 01期

关键词：

D O I：

10.1094/Phyto-85-87

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

High yields of conidia of Venturia inaequalis were produced from either mycelial fragments or conidia on cellophane-covered surfaces of agar media incubated under continuous near-ultraviolet light. Optimal conditions required a cellophane-covered surface of potato-dextrose agar in combination with light and incubation for 1 wk. The procedure represents a convenient techique for the mass-production of clonal and sterile V. inaequalis conidia. Conidia were biolistically transformed to hygromycin B resistance with a plasmid (pOHT) containing a bacterial phosphotransferase gene spliced between regulatory elements from Aspergillus nidulans. Southern hybridization analysis showed that the plasmid was incorporated into heterologous regions of the genome. Hygromycin B-resistant transformants were mitotically stable during nonselective propagation. The heterologous gene conferring hygromycin B resistance was expressed during early stages of conidia germination and during vegetative growth of mycelium.

引用

页码：87 / 91

页数：5

共 34 条

[1]

BOONE DM, 1988, ADV PLANT PATHOL, V6, P249

[2]

BRAY MYRTLE F., 1962, PLANT PATHOL, V11, P106, DOI 10.1111/j.1365-3059.1962.tb00176.x

[3] TRANSFORMATION OF 7 SPECIES OF FILAMENTOUS FUNGI USING THE NITRATE REDUCTASE GENE OF ASPERGILLUS-NIDULANS [J].