CELL-CYCLE MAPPING BY IRRADIATING CELLS WITH BROMOSUBSTITUTED DNA SEGMENTS

被引：12

作者：

DELATORRE, C

GONZALEZFERNANDEZ, A

机构：

[1] Instituto de Biologia Celular (C.S.I.C.), Madrid, 144, Velazquez

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1979年 / 29卷 / 05期

关键词：

D O I：

10.1111/j.1751-1097.1979.tb07801.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract—The role of chromatin replicated at restricted S intervals in further interphase progression has been analyzed by bromosubstitution followed by black light irradiation (313 nm peak) in a naturally synchronous population rendered binucleate by a caffeine pulse in Allium cepa L. root meristems. Interphase completion is preferentially halted when irradiating cells with bromosubstituted chromatin which is replicated at the 3rd and 4th interval of an S period artificially divided into 5 portions. The treatment apparently inhibits transcription of the bromosubstituted DNA segments. The failure in the transcription of these segments may be related to the transition point located at early G2 in these cells. where protein synthesis is needed in order to reach mitosis. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：977 / 981

页数：5

共 24 条

[1] REGULATION OF CYCLE PROGRESSION IN PLANT-CELLS [J].