CONSTRUCTION AND HYPERPRODUCTIVITY OF ENGINEERED STRAIN QE79 BEARING RECOMBINANT PLASMID CONTAINING PENICILLIN-G ACYLASE GENE FROM ESCHERICHIA-COLI STRAIN AS1.76

被引：9

作者：

ZHANG, QJ ^{[1
]}

ZHANG, LF ^{[1
]}

LIU, Q ^{[1
]}

HAN, JH ^{[1
]}

CHEN, LX ^{[1
]}

机构：

[1] CTR DEV BIOTECHNOL,YICHANG,PEOPLES R CHINA

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 07期

关键词：

D O I：

10.1007/BF01086341

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An engineered strain QE79 bearing a recombinant plasmid containing the penicillin G acylase gene from E.coli strain AS1.76 was constructed. Formation conditions of the penicillin G acylase were studied. The activity of the enzyme reached over 200 units per 100ml of the culture when the strain QE79 was grown in the medium consisting of mineral salts with supplementary glucose or sucrose at 28°C for 32 hr on shaker. The productivity of the engineered strain QE79 was nearly nine times higher than that of the original strain. © 1990 Kluwer Academic Publishers.

引用

页码：493 / 498

页数：6

共 13 条

[1]

CASADABAM MJ, 1980, J MOL BIOL, V138, P207

[2] ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI [J].