THE BINDING-SITE OF THE TRANSCRIPTIONAL ACTIVATOR VIRG FROM AGROBACTERIUM COMPRISES BOTH CONSERVED AND SPECIFIC NONCONSERVED SEQUENCES

被引：8

作者：

ROITSCH, T ^{[1
]}

JIN, S ^{[1
]}

NESTER, EW ^{[1
]}

机构：

[1] UNIV REGENSBURG,LEHRSTUHL ZELLBIOL & PFLANZENPHYSIOL,D-93040 REGENSBURG,GERMANY

来源：

FEBS LETTERS | 1994年 / 338卷 / 02期

关键词：

AGROBACTERIUM; VIRULENCE; VIR GENE INDUCTION; VIRG;

D O I：

10.1016/0014-5793(94)80349-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Virulence genes of Agrobacterium tumefaciens are transcriptionally activated in response to phenolic compounds and certain sugars. The transcriptional activator VirG specifically binds to fragments containing the conserved vir box sequence present in the promoter region of all vir genes. This study shows that both the vir box as well as specific nonconserved sequences downstream of the vir box are required for VirG binding and transcriptional activation. Insertion of the identified VirG binding site into the lac promoter resulted in transcriptional activation of this heterologous promoter in response to the plant phenolic signal molecule acetosyringone.

引用

页码：127 / 132

页数：6

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