ORIENTATED MATS OF FIBRONECTIN AS A CONDUIT MATERIAL FOR USE IN PERIPHERAL-NERVE REPAIR

被引：87

作者：

WHITWORTH, IH

BROWN, RA

DORE, C

GREEN, CJ

TERENGHI, G

机构：

[1] From the Blond-McIndoe Research Centre, Queen Victoria Hospital, East Grinstead, the Phoenix Department of Plastic Surgery, The Rayne Institute, University College Hospital, London

[2] Northwick Park Institute for Medical Research, Northwick Park Hospital, Harrow, Middlesex

来源：

JOURNAL OF HAND SURGERY-BRITISH AND EUROPEAN VOLUME | 1995年 / 20B卷 / 04期

关键词：

D O I：

10.1016/S0266-7681(05)80148-2

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

This study introduces a new nerve conduit material consisting of orientated strands of the cell adhesive fibronectin. Axonal regeneration, Schwann cell behaviour and the degree of inflammation were quantified using immunohistochemical techniques and computerized image analysis, The results when fibronectin was used to bridge a 1 cm defect in rat sciatic nerve were compared to those with autologous nerve grafts and freeze-thawed muscle grafts used as controls. The nerve grafts supported the highest rate and amount of axonal regeneration in the first 10 days; however, the fibronectin supported a significantly faster rate of growth (P=0.0008) and amount (P=0.0001) of axons than the freeze-thawed muscle grafts, From day 15 onwards, the fibronectin and nerve grafts had comparable amounts of regenerating axons and Schwann cells. We conclude that the orientated form of fibronectin is a suitable material for successful nerve repair and has potential clinical use.

引用

页码：429 / 436

页数：8

共 52 条

[1]

Abercrombie, The crawling movement of metazoan cells, Proceedings of the Royal Society B: Biological Sciences, 207 B, pp. 129-147, (1950)

[2]

Adams, Watt, Expression of beta 1, beta 3, beta 4 and beta 5 integrins by human epithelial keratinocytes and non-differentiating keratinocytes, Journal of Cell Biology, 115, pp. 829-841, (1991)

[3]

Akers, Mosher, Lilien, Promotion of retinal neurite out-growth by substratum-bound fibronectin, Developmental Biology, 86, pp. 179-188, (1981)

[4]

Bailey, Eichler, Villadiego, Rich, The influence of fibronectin and laminin during Schwann cell migration and peripheral nerve regeneration through silicon chambers, Journal of Neurocytology, 22, pp. 176-184, (1993)

[5]

Baron-van Evercooren, Kleinman, Ohno, Marangos, Schwartz, Dubois-Dalcq, Nerve growth factor, laminin, and fibronectin promote neurite growth in human fetal sensory ganglia culture, Journal of Neuroscience Research, 8, pp. 179-193, (1982)

[6]

Baron-van Evercooren, Kleinman, Seppa, Rentier, Dubois-Dalcq, Fibronectin promotes rat Schwann cell growth and motility, Journal of Cell Biology, 93, pp. 211-216, (1982)

[7]

Bignami, Chi, Dahl, Laminin in rat sciatic nerve undergoing Wallerian degeneration, Journal of Neuropathology and Experimental Neurology, 43, 1, pp. 94-103, (1984)

[8]

Brown, Perry, Lunn, Gordon, Heumann, Macrophage dependence of peripheral sensory nerve regeneration: Possible involvement of nerve growth factor, Neuron, 6, pp. 359-370, (1991)

[9]

Brown, Blunn, Ejim, Preparation of orientated fibrous mats from fibronectin: Composition and stability, Biomaterials, 15, pp. 457-464, (1994)

[10]

Brunelli, Fontana, Jager, Bartolaminelli, Franchini, Chemotactic arrangement of axons inside and distal to a venous graft, Journal of Reconstructive Microsurgery, 3, pp. 87-89, (1987)

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