PROPOSED MECHANISM FOR MICROBIAL-DEGRADATION OF POLYACRYLATE

被引:7
作者
KAWAI, F
机构
来源
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY | 1995年 / A32卷 / 04期
关键词
D O I
10.1080/10601329508010293
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
A possible aerobic degradation mechanism for polyacrylate (PA) was examined with acrylic oligomer-utilizing bacteria (Microbacterium sp., Xanthomonas maltophilia, and Acinetobacter sp.), using a model compound (1,3,5-pentane tricarboxylic acid, PTCA). Acyl-coenzyme A synthetase activities were detected with dialyzed cell-free extracts of PTCA-utilizing bacteria toward PTCA, PA 500, and PA 1000. This result suggested that PA is activated by coenzyme A and metabolized via PA-coenzyme A. Metabolic products formed from PTCA were detected in culture filtrates and reaction mixtures of washed cells. Fraction A was detected as a main metabolite by high-performance liquid chromatography. A small amount of fraction B was concomitant with fraction A. Also, another fraction, C,was detected. These intermediate metabolites were characterized by LC-MS as 1,3,5-(1- or 2-pentene)tricarboxylic acid for fractions A and B and as 1,3,5-(2-oxopentane)tricarboxylic acid for fraction C. Fraction A was metabolized far faster than fraction B. Fraction B was thought to be an artifact formed from fraction A under alkaline conditions. Thus PTCA and also PA seemed to be metabolized by the mechanism similar to P-oxidation of fatty acids. The degradation of PTCA by washed cells was slower than that by growing cells and was inhibited by 5 mM NaN3,. This suggests that the metabolism is linked to a respiratory chain of bacteria.
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页码:835 / 838
页数:4
相关论文
共 2 条
[1]  
KAWAI F, 1993, APPL MICROBIOL BIOT, V39, P382
[2]  
Kawai Fusako, 1994, Journal of Environmental Polymer Degradation, V2, P59, DOI 10.1007/BF02074774