SOLID-PHASE EXTRACTION AND LIQUID-CHROMATOGRAPHY OF TORSEMIDE AND METABOLITES FROM PLASMA AND URINE

被引：36

作者：

MARCH, C ^{[1
]}

FARTHING, D ^{[1
]}

WELLS, B ^{[1
]}

BESENFELDER, E ^{[1
]}

KARNES, HT ^{[1
]}

机构：

[1] BOEHRINGER MANNHEIM GMBH,DEPT BIOANALYT,W-6800 MANNHEIM 31,GERMANY

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 1990年 / 79卷 / 05期

关键词：

D O I：

10.1002/jps.2600790520

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Torsemide is a new diuretic drug with a profile of action similar to that of furosemide. The high potency of torsemide results in low dose therapy and causes problems for the pharmacokinetic study of the drug due to low plasma levels. Described here are methods for the analysis of torsemide and two metabolites in plasma and urine using solid‐phase extraction and liquid chromatography. The limits of quantitation are 10 ng/mL for plasma and 20 ng/mL for urine. The relative standard deviations for precision are <10% for most analytes at most concentrations in the calibration range. The recoveries from plasma were 94.3, 92.9, and 95.6%, and from urine were 77.5, 66.6, and 76.5% for torsemide and metabolites M1 and M5, respectively. The method was suitable for pharmacokinetic studies. Data from a normal volunteer are presented. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：453 / 457

页数：5