ORGANIZATION OF THE REPLICATION CONTROL REGION OF PLASMID COLIB-P9

被引：39

作者：

HAMA, C ^{[1
]}

TAKIZAWA, T ^{[1
]}

MORIWAKI, H ^{[1
]}

URASAKI, Y ^{[1
]}

MIZOBUCHI, K ^{[1
]}

机构：

[1] UNIV TOKYO,FAC SCI,DEPT BIOPHYS & BIOCHEM,TOKYO 113,JAPAN

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 04期

关键词：

D O I：

10.1128/jb.172.4.1983-1991.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We identified a 1,845-base-pair sequence that contains essential information for the autonomous replication and regulation of the 93-kilobase-pair IncIα group ColIb-P9 plasmid. Biochemical and gentic analyses revealed that this sequence specifies at least two structural genes, designated repZ and inc. The repZ gene encodes a protein with a molecular weight of 39,000, which probably functions as an initiator for the ColIb-P9 replicon. The inc gene that phenotypically governs the incompatibility encodes an RNA with a size of about 70 bases. This small RNA acts in trans to repress the expression of repZ, thereby functioning to maintain a constant copy number of the ColIb-P9 replicon in host cells.

引用

页码：1983 / 1991

页数：9

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