HISTOCHEMICAL-CHARACTERISTICS OF DIPHOSPHATE NUCLEOSIDE CONSUMPTION IN CAT AND RAT NERVOUS-SYSTEM

被引:10
作者
MARTINEZMURILLO, R
MARTINEZRODRIGUEZ, R
TOLEDANO, A
BARCA, MA
机构
[1] Instituto Cajal, Madrid
关键词
NDP; NDP-ase; Nucleoside diphosphatase; Nucleoside diphosphate; Thiaminepyrophosphatase; Thiaminepyrophosphate; TPP; TPP-ase;
D O I
10.1016/S0065-1281(79)80002-1
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Consumption of diphosphate nucleosides is investigated by histochemical methods in rat and cat nervous system. Results show NDP-ase is effectively in histological sections from animals previously perfused with glutaraldehyde or formaldehyde. Histochemical reaction is increased in presence of Ca++, Mg++, Mn++ or imidazole and inhibited by L-DOPA and Noradrenaline in incubation medium. A comparative study of TPP-ase and NDP-ase activities to dilucidate the identity of both enzymes is described. © 1979, VEB Gustav Fischer Verlag Jena. All rights reserved.
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页码:138 / 145
页数:8
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