VISUALIZATION OF SINGLE MOLECULES OF RNA-POLYMERASE SLIDING ALONG DNA

被引：233

作者：

KABATA, H

KUROSAWA, O

ARAI, I

WASHIZU, M

MARGARSON, SA

GLASS, RE

SHIMAMOTO, N

机构：

[1] NATL INST GENET,DNA RES CTR,MISHIMA,SHIZUOKA 411,JAPAN

[2] GRAD UNIV ADV STUDIES,SCH LIFE SCI,MISHIMA 411,JAPAN

[3] SEIKEI UNIV,DEPT ELECT ENGN & ELECTR,TOKYO 103,JAPAN

[4] ADVANCE CO,TOKYO 103,JAPAN

[5] UNIV NOTTINGHAM HOSP,QUEENS MED CTR,DEPT BIOCHEM,NOTTINGHAM NG7 2UH,ENGLAND

来源：

SCIENCE | 1993年 / 262卷 / 5139期

关键词：

D O I：

10.1126/science.8248804

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transcription requires that RNA polymerase binds to promoters buried in nonspecific sites on DNA. The search for promoters may be facilitated if the polymerase slides along the molecule of DNA. Single molecules of Escherichia coli RNA polymerase were visualized, and their movements on immobilized bacteriophage lambda and T7 DNAs were examined. Deviating from drifts by bulk flow, about 40 percent of the enzyme molecules moved along the extended DNA. The results provide direct evidence for sliding as a mechanism for relocation of the enzyme on DNA.

引用

页码：1561 / 1563

页数：3

共 31 条

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