EXON SKIPPING BY OVEREXPRESSION OF A DROSOPHILA HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN IN-VIVO

被引：21

作者：

SHEN, J

ZU, K

CASS, CL

BEYER, AL

HIRSH, J

机构：

[1] UNIV VIRGINIA,DEPT BIOL,CHARLOTTESVILLE,VA 22903

[2] UNIV VIRGINIA,DEPT MICROBIOL,CHARLOTTESVILLE,VA 22903

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 06期

关键词：

ALTERNATIVE SPLICING; DOPA DECARBOXYLASE;

D O I：

10.1073/pnas.92.6.1822

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are abundant RNA-binding proteins that are implicated in splicing regulation, Here we investigate the role of a Drosophila hnRNP in splicing regulation in living animals. We find that overexpression of the Drosophila hnRNP HRB98DE leads to skipping of all internal exons in the Drosophila dopa decarboxylase (Ddc) pre-mRNA in vivo. These results indicate that HRB98DE has a splicing activity that promotes use of terminal splice sites. The effect of excess HRB98DE on Ddc splicing is transient, even though high levels of HRB98DE persist for at least 24 hr. This suggests that Drosophila larvae can induce a compensating mechanism to counteract the effects of excess HRB98DE.

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页码：1822 / 1825

页数：4

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