INVOLVEMENT OF RAINBOW-TROUT LEUKOCYTES IN THE PATHOGENESIS OF INFECTIOUS HEMATOPOIETIC NECROSIS

被引：11

作者：

CHILMONCZYK, S ^{[1
]}

WINTON, JR ^{[1
]}

机构：

[1] NATL FISHERIES RES CTR,NAVAL STN,SEATTLE,WA 98115

来源：

DISEASES OF AQUATIC ORGANISMS | 1994年 / 19卷 / 02期

关键词：

LEUKOCYTES; RAINBOW TROUT; IHNV;

D O I：

10.3354/dao019089

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Rainbow trout Oncorhynchus mykiss leucocytes were tested for their ability to support replication of infectious hematopoietic necrosis virus (IHNV). Viral replication occurred in vitro using leucocytes cultured from peripheral blood, kidney, and thymus where viral titers peaked at 2 to 4 d post-inoculation. Leucocytes collected from trout following waterborne challenge with IHNV were cocultured on EPC cell monolayers. These assays detected IHNV in leucocytes infected in vivo as early as 6 h post-exposure before the challenge virus had undergone replication. These data showed that leucocyte populations could serve as target cells in the initial phase of IHNV infection.

引用

页码：89 / 94

页数：6