COMPREHENSIVE MOLECULAR MAP OF BACTERIOPHAGE-LAMBDA

被引：289

作者：

SZYBALSKI, EH

SZYBALSKI, W

机构：

[1] McArdle Laboratory for Cancer Research, University of Wisconsin, Madison

来源：

GENE | 1979年 / 7卷 / 3-4期

关键词：

deletions; DNA strands; Gene products; insertions; phages; recognition sites; restriction endonucleases; substitutions; transcripts; transducing; transposons;

D O I：

10.1016/0378-1119(79)90047-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Physical and genetic mapping of deletion mutations has been correlated with the available molecular sizes of the λ gene products and the DNA base sequence to construct a comprehensive molecular map of the phage λ genome. The physical length of the DNA making up the left arm from the cos site through gene J is not sufficient to account in a nonoverlapping manner for all the proteins of the sizes reported to be coded, especially in the Nu1-C region. In the right arm all the coding capacity has not been accounted for, and it appears to be oversaturated only in the gam-ral region. The positions of several IS and Tn elements, and of restriction endonuclease cleavage sites are specified. © 1979.

引用

页码：217 / 270

页数：54

共 365 条

[1] RELEASE OF POLARITY IN ESCHERICHIA-COLI BY GENE N OF PHAGE LAMBDA - TERMINATION AND ANTITERMINATION OF TRANSCRIPTION [J].