DNA-SEQUENCE ORGANIZATION IN THE ALGA EUGLENA-GRACILIS

被引：12

作者：

RAWSON, JRY ^{[1
]}

ECKENRODE, VK ^{[1
]}

BOERMA, CL ^{[1
]}

CURTIS, S ^{[1
]}

机构：

[1] UNIV GEORGIA,BIOCHEM,ATHENS,GA 30602

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 563卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

(Euglena gracilis); DNA sequence organization; Genome complexity; Repetitive DNA sequence;

D O I：

10.1016/0005-2787(79)90002-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence organization of nuclear DNA in the single-celled alga Euglena gracilis has been studied by a combination of techniques: (1) the comparison of the reassociation kinetics of DNA fragments 300, 2000 and 8100 nucleotides long; (2) the reassociation of 32P-labeled DNA fragments of various lengths with driver fragments 300 nucleotides long; (3) the hyperchromicity of DNA structures formed by the reassociation of repetitive sequences; (4) and the direct measurement of the size of the duplex regions of reassociated repetitive DNA resistant to S1 nuclease. The single copy DNA sequences are approximately 1500 nucleotide pairs long and are interspersed with repetitive DNA sequences. The repetitive DNA, consisting of both highly repetitive and middle repetitive sequences, consists of one fraction of nucleotide sequences (0.67) with an average size of 4900 nucleotide pairs and a second fraction (0.33) with an average size of 1000 nucleotide pairs. 34% of the DNA consists of foldback sequences which are present on 45% of the DNA 4000 nucleotides long. © 1979.

引用

页码：1 / 16

页数：16

共 30 条

[1] REDUCTION IN RATE OF DNA REASSOCIATION BY SEQUENCE DIVERGENCE [J].